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Western blot(SDS PAGE) analysis of extracts from (1) HeLa cell lysate; (2) NIH 3T3 cell lysate; (3) C6 cell lysate; (4) Zebrafish lysates; (5) COS-1 cell lysate; (6) MDCK cell lysate.Using Tubulin gamma Rabbit mAb [UK8a]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Gamma-1-tubulin; Gamma-tubulin complex component 1; GCP-1; TBG1; TUBG; TUBG1; TUBGCP1; Tubulin gamma-1 chain; tubulin, gamma 1; tubulin, gamma polypeptide,gamma tubulin;
UniProtID :P23258
MASS(da) :51,170
MW(kDa) :48kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat Monkey Chicken Hamster Cow Dog Fish Xenopus tropicalis
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Tubulin gamma
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1) HeLa cell lysate; (2) NIH 3T3 cell lysate; (3) C6 cell lysate; (4) Zebrafish lysates; (5) COS-1 cell lysate; (6) MDCK cell lysate.
Function : Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation.
Subcellular locationi :Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.