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Western blot(SDS PAGE) analysis of extracts from (1) C6 cell lysate; (2) Jurkat cell lysate.Using Ras Rabbit mAb [uBIe]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :GTPase HRas;GTPase KRas;GTPase NRas;H-Ras-1;HAMSV;HRAS;HRAS1;K-RAS;N-RAS;NRAS;p21ras;RASH1;RASK2;Transforming protein p21
UniProtID :P01111
MASS(da) :21,229
MW(kDa) :18kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Ras
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1) C6 cell lysate; (2) Jurkat cell lysate.
Function : Ras proteins bind GDP/GTP and possess intrinsic GTPase activity..
Subcellular locationi :Cell membrane,Lipid-anchor,Cytoplasmic side. Golgi apparatus membrane,Lipid-anchor.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.