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Western blot(SDS PAGE) analysis of extracts from (1)HepG2 cell lysate; (2)JAR cell lysate.Using Alkaline Phosphatase Rabbit mAb [H0WJ]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Alkaline phosphatase, tissue-nonspecific isozyme; AP-TNAP; TNSALP; Alkaline phosphatase liver/bone/kidney isozyme; ALPL;
UniProtID :P05186
MASS(da) :57,305
MW(kDa) :70 kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Alkaline Phosphatase
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1)HepG2 cell lysate; (2)JAR cell lysate.
Subcellular locationi :Cell membrane,Lipid-anchor, GPI-anchor. Extracellular vesicle membrane,Lipid-anchor, GPI-anchor. Mitochondrion membrane,Lipid-anchor, GPI-anchor. Mitochondrion intermembrane space.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.