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Western blot(SDS PAGE) analysis of extracts fromHeLa cell lysate treated Trichostatin A.Using Histone H2A (acetyl K9) Rabbit mAb [hj8u]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :H2A; H2A1B; H2AFM; HIST1H2A; Histone H2A.2; Histone H2A/a;
UniProtID :P04908
MASS(da) :14,135
MW(kDa) :14kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Histone H2A (acetyl K9)
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected : HeLa cell lysate treated Trichostatin A.
Function : Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Subcellular locationi :Nucleus. Chromosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.