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P-Histone H1.4 (T17) Rabbit mAb [bzqJ]Cat NO.: A58074

Western blot(SDS PAGE) analysis of extracts from Jurkat cell lysate.Using P-Histone H1.4 (T17) Rabbit mAb [bzqJ]at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :Histone H1b; Histone H1s-4;

UniProtID :P10412

MASS(da) :21,865

MW(kDa) :30kDa

Form :Liquid

Purification :Affinity-chromatography

Host :Rabbit

Isotype : IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Phospho-Histone H1.4 (T17)

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Jurkat cell lysate.

Function : Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity)..

Subcellular locationi :Nucleus. Chromosome.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.