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Western blot(SDS PAGE) analysis of extracts from SH-SY5Y cell lysate.Using PI 3 Kinase p55 gamma Rabbit mAb [5Q1G]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :GRB1, P85A, PI3-kinase p85-alpha subunit, PI3K, PI3K p85-alpha; P55G, PI3-kinase p85-gamma subunit, PI3K p85-gamma,
UniProtID :Q92569
MASS(da) :54,448
MW(kDa) :54kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human PI 3 Kinase p55 gamma
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :SH-SY5Y cell lysate.
Function : Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1.
Tissue specificity :Highest levels in brain and testis. Lower levels in adipose tissue, kidney, heart, lung and skeletal muscle.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.