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EEA1 Rabbit mAb [n9t4]Cat NO.: A28181

Western blot(SDS PAGE) analysis of extracts from (1) HeLa cell lysate; (2) NIH/3T3 cell lysate.Using EEA1 Rabbit mAb [n9t4]at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :Early endosome antigen 1; EEA1; MST105; ZFYVE2;

UniProtID :Q15075

MASS(da) :162,466

MW(kDa) :170kDa

Form :Liquid

Purification :Affinity-chromatography

Host :Rabbit

Isotype : IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫荧光(ICC/IF)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human EEA1

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :(1) HeLa cell lysate; (2) NIH/3T3 cell lysate.

Function : Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.

Subcellular locationi :Cytoplasm. Early endosome membrane,Peripheral membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.