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Western blot(SDS PAGE) analysis of extracts from THP-1 cell lysate.Using TPH1 Rabbit mAb [AUtN]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TPH1;MGC119994;TPRH;TRPH;Tryptophan 5-hydroxylase 1; Tryptophan Hydroxylase;
UniProtID :P17752
MASS(da) :50,985
MW(kDa) :51kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human TPH1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :THP-1 cell lysate.
Function : Oxidizes L-tryptophan to 5-hydroxy-l-tryptophan in the rate-determining step of serotonin biosynthesis..
Tissue specificity :[Isoform 2]: Seems to be less widely expressed than isoform 1..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.