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Western blot(SDS PAGE) analysis of extracts from SH-SY5Y cell lysate.Using GAP43 Rabbit mAb [nGM4]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :GAP43, Axonal membrane protein GAP-43, B-50, Growth associated protein 43, Neural phosphoprotein B-50, Neuromodulin, PP46, GAP-43, Protein F1;
UniProtID :P17677
MASS(da) :24,803
MW(kDa) :48kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human GAP43
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :SH-SY5Y cell lysate.
Function : This protein is associated with nerve growth. It is a major component of the motile 'growth cones' that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction..
Subcellular locationi :Cell membrane,Peripheral membrane protein,Cytoplasmic side. Cell projection, growth cone membrane,Peripheral membrane protein,Cytoplasmic side. Cell junction, synapse. Cell projection, filopodium membrane,Peripheral membrane protein. Perikaryon. Cell projection, dendrite. Cell projection, axon. Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.