eIF5A Rabbit mAb [u64o]Cat NO.: A65392

Protein names ：EIF-5A; EIF5A1; eIF5AI; Eukaryotic initiation factor 5A;

UniProtID ：P63241

MASS(da) ：16,832

MW(kDa) ：18kDa

Form ：Liquid

Purification ：Affinity-chromatography

Host ：Rabbit

Isotype ： IgG

sensitivity ：Endogenous

Reactivity ：Human,Mouse,Rat

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• 免疫组化(IHC)1:100
• 免疫荧光(ICC/IF)1:100
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with A synthesized peptide derived from human eIF5A

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：Jurkat cell lysate.

Function ： mRNA-binding protein involved in translation elongation (PubMed:16987817, PubMed:17187778, PubMed:33547280). Has an important function at the level of mRNA turnover, probably acting downstream of decapping (PubMed:16987817, PubMed:17187778, PubMed:33547280). Critical for the efficient synthesis of peptide bonds between consecutive proline residues (PubMed:16987817, PubMed:17187778, PubMed:33547280). Can resolve ribosomal stalling caused by consecutive prolines during translation (PubMed:16987817, PubMed:17187778, PubMed:33547280). Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity (PubMed:16987817, PubMed:17187778, PubMed:33547280). With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis (PubMed:15371445). Regulates also TNF-alpha-mediated apoptosis (PubMed:15452064). Mediates effects of polyamines on neuronal process extension and survival (PubMed:17360499). May play an important role in brain development and function, and in skeletal muscle stem cell differentiation (By similarity). Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts (PubMed:8253832)..

Tissue specificity ：Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level)..

Subcellular locationi ：Cytoplasm. Nucleus. Endoplasmic reticulum membrane,Peripheral membrane protein,Cytoplasmic side.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.