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Western blot(SDS PAGE) analysis of extracts from HeLa cell lysate.Using Radixin Rabbit mAb [B0Lo]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :RDX; Radixin; Protein roadkill; HIB; ESP10; DFNB24; RADI;
UniProtID :P35241
MASS(da) :68,564
MW(kDa) :80kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Radixin
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cell lysate.
Function : Probably plays a crucial role in the binding of the barbed end of actin filaments to the plasma membrane.
Subcellular locationi :Cell membrane,Peripheral membrane protein,Cytoplasmic side. Cytoplasm, cytoskeleton. Cleavage furrow. Cell projection, microvillus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.