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NSE Rabbit mAb [ni2z]Cat NO.: A91217

Western blot(SDS PAGE) analysis of extracts from HeLa cell lysate.Using NSE Rabbit mAb [ni2z]at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :ENO2; ENOG; Enolase 2; enolase 2 (gamma, neuronal); Gamma-enolase; Neural enolase; neuron specific gamma enolase; Neuron-specific enolase; neurone-specific enolase; NSE;

UniProtID :P09104

MASS(da) :47,269

MW(kDa) :47kDa

Form :Liquid

Purification :Affinity-chromatography

Host :Rabbit

Isotype : IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human NSE

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cell lysate.

Function : Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity)..

Tissue specificity :The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

Subcellular locationi :Cytoplasm. Cell membrane.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.