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Western blot(SDS PAGE) analysis of extracts from (1) HeLa cell lysate; (2) Hela cells lysate treated with AP.Using P-SMC1 (S957) Rabbit mAb [5465]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SB1.8/DXS423E protein; SM1A; SMC1A; SMC1L1; SMC1alpha protein; Sb1.8;
UniProtID :Q14683
MASS(da) :143,233
MW(kDa) :160kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Phospho-SMC1 (S957)
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1) HeLa cell lysate; (2) Hela cells lysate treated with AP.
Function : Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint..
Subcellular locationi :Nucleus. Chromosome. Chromosome, centromere, kinetochore.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.