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Western blot(SDS PAGE) analysis of extracts from HeLa cell lysate.Using P Glycoprotein Rabbit mAb [Tv39]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CD243; MDR1; MDR3; Multidrug resistance protein 1; P-glycoprotein 1; PGY1;
UniProtID :P08183
MASS(da) :141,479
MW(kDa) :180kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human P Glycoprotein
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cell lysate.
Function : Translocates drugs and phospholipids across the membrane (PubMed:8898203, PubMed:2897240, PubMed:9038218). Catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Participates mainly to the flop of phosphatidylcholine, phosphatidylethanolamine, beta-D-glucosylceramides and sphingomyelins (PubMed:8898203). Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells (PubMed:2897240, PubMed:9038218)..
Tissue specificity :Expressed in liver, kidney, small intestine and brain.
Subcellular locationi :Cell membrane,Multi-pass membrane protein. Apical cell membrane.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.