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Western blot(SDS PAGE) analysis of extracts from Jurkat cell lysate.Using IKB beta Rabbit mAb [5Nfn]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :I-kappa-B-beta; IkB-B; IkB-beta; IKBB; NF-kappa-BIB; NFKBIB; Thyroid receptor-interacting protein 9; TR-interacting protein 9; TRIP;
UniProtID :Q15653
MASS(da) :37,771
MW(kDa) :48kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human IKB beta
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Jurkat cell lysate.
Function : Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.
Tissue specificity :Expressed in all tissues examined.
Subcellular locationi :Cytoplasm. Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.