GARS Rabbit mAb [1i9m]Cat NO.: A85947

Protein names ：HMN5; CMT2D; DSMAV; GlyRS; SMAD1;

UniProtID ：P41250

MASS(da) ：83,166

MW(kDa) ：75kDa

Form ：Liquid

Purification ：Affinity-chromatography

Host ：Rabbit

Isotype ： IgG

sensitivity ：Endogenous

Reactivity ：Human,Mouse,Rat

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• 免疫组化(IHC)1:100
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with A synthesized peptide derived from human GARS

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：(1) Jurkat cell lysate; (2) 293T cell lysate.

Function ： Catalyzes the ATP-dependent ligation of glycine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (Gly-AMP) (PubMed:17544401, PubMed:28675565, PubMed:24898252). Also produces diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs. Thereby, may play a special role in Ap4A homeostasis (PubMed:19710017)..

Tissue specificity ：Widely expressed, including in brain and spinal cord..,[Isoform 2]: Expressed in brain, spinal cord, muscle, heart and spleen..,[Isoform 1]: Expressed in brain, spinal cord, muscle, heart, spleen and liver..

Subcellular locationi ：Cytoplasm. Cell projection, axon. Secreted. Secreted, extracellular exosome.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.