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Western blot(SDS PAGE) analysis of extracts from HeLa cell lysate treated with staurosporine.Using cleaved Caspase-9 Rabbit mAb [nbgx]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6; CASP9; Caspase-9; Caspase 9 Dominant Negative;
UniProtID :P55211
MASS(da) :46,281
MW(kDa) :46,39,37,35kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human Mouse
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human cleaved Caspase-9
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cell lysate treated with staurosporine.
Function : Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP)., Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
Tissue specificity :Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.