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AKAP14 Rabbit mAb [2B2e]Cat NO.: A85263

Western blot(SDS PAGE) analysis of extracts from (1) Jurkat cell lysate; (2) A549 cell lysate.Using AKAP14 Rabbit mAb [2B2e]at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :A-kinase anchor protein 14; A-kinase anchor protein 14; A-kinase anchor protein 28 kDa; AKAP14; AKAP28;

UniProtID :Q86UN6

MASS(da) :22,815

MW(kDa) :23kDa

Form :Liquid

Purification :Affinity-chromatography

Host :Rabbit

Isotype : IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫荧光(ICC/IF)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human AKAP14

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :(1) Jurkat cell lysate; (2) A549 cell lysate.

Function : Binds to type II regulatory subunits of protein kinase A and anchors/targets them.

Tissue specificity :Present in cilia (at protein level). Expressed in tissues containing axoneme-based organelles (cilia and/or flagella): trachea and testis. Highly expressed in airway cilia..

Subcellular locationi :Cytoplasm.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.