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Western blot(SDS PAGE) analysis of extracts from (1) Jurkat cell lysate; (2) A549 cell lysate.Using AKAP14 Rabbit mAb [2B2e]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :A-kinase anchor protein 14; A-kinase anchor protein 14; A-kinase anchor protein 28 kDa; AKAP14; AKAP28;
UniProtID :Q86UN6
MASS(da) :22,815
MW(kDa) :23kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human AKAP14
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1) Jurkat cell lysate; (2) A549 cell lysate.
Function : Binds to type II regulatory subunits of protein kinase A and anchors/targets them.
Tissue specificity :Present in cilia (at protein level). Expressed in tissues containing axoneme-based organelles (cilia and/or flagella): trachea and testis. Highly expressed in airway cilia..
Subcellular locationi :Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.