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Western blot(SDS PAGE) analysis of extracts from Mouse heart lysate.Using Myosin Light Chain 2 Rabbit mAb [2Gbv]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MYL2; MLC2; Myosin light chain 2; MLC-2; RLC of myosin; CMH10; MLC-2v;
UniProtID :P10916
MASS(da) :18,789
MW(kDa) :18kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Myosin Light Chain 2
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Mouse heart lysate.
Function : Contractile protein that plays a role in heart development and function (PubMed:23365102, PubMed:32453731). Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion,as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force (By similarity). During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly (By similarity)..
Tissue specificity :Highly expressed in type I muscle fibers..
Subcellular locationi :Cytoplasm, myofibril, sarcomere, A band.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.