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Western blot(SDS PAGE) analysis of extracts from 293T cell lysate.Using VAV2 Rabbit mAb [7K5n]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :VAV2; Vav 2 oncogene; VAV-2;
UniProtID :P52735
MASS(da) :101,289
MW(kDa) :101kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human VAV2
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :293T cell lysate.
Function : Guanine nucleotide exchange factor for the Rho family of Ras-related GTPases. Plays an important role in angiogenesis. Its recruitment by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity)..
Tissue specificity :Widely expressed.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.