* 产品详情- */>
Western blot(SDS PAGE) analysis of extracts from HeLa cell lysate.Using Dopamine Transporter Rabbit mAb [Ymer]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :DA transporter; DAT1; Dopamine transporter 1; PKDYS; SLC6A3;
UniProtID :Q01959
MASS(da) :68,495
MW(kDa) :50kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Dopamine Transporter
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cell lysate.
Function : Amine transporter (PubMed:1406597, PubMed:8302271, PubMed:15505207). Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals (By similarity). Regulator of light-dependent retinal hyaloid vessel regression, downstream of OPN5 signaling (By similarity)..
Tissue specificity :Highly expressed in substantia nigra (PubMed:7637582). Expressed in axonal varicosities in dopaminergic nerve terminals (at protein level) (PubMed:17296554). Expressed in the striatum (at protein level) (PubMed:17296554)..
Subcellular locationi :Cell membrane,Multi-pass membrane protein. Cell projection, neuron projection. Cell projection, axon.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.