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Carbonic Anhydrase 9 (CA9/G250) Rabbit mAb[S2DL]Cat NO.: A41103

Western blot(SDS PAGE) analysis of extracts from HT-29 cells.Using Carbonic Anhydrase 9 (CA9/G250) Rabbit mAb IgG [S2DL] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :CA9,G250,MN,CAH9_HUMAN,Carbonic anhydrase 9

UniProtID :Q16790

MASS(da) :49,698

MW(kDa) :46,50,58KDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000,
  • 免疫组化(IHC)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Carbonic Anhydrase 9 (CA9/G250).

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HT-29 cells

Function : Catalyzes the interconversion between carbon dioxide and water and the dissociated ions of carbonic acid (i.e. bicarbonate and hydrogen ions)..

Tissue specificity :Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.

Subcellular locationi :Nucleus. Nucleus, nucleolus. Cell membrane,Single-pass type I membrane protein. Cell projection, microvillus membrane,Single-pass type I membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.