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Western blot(SDS PAGE) analysis of extracts from HT-29 cells.Using Carbonic Anhydrase 9 (CA9/G250) Rabbit mAb IgG [S2DL] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CA9,G250,MN,CAH9_HUMAN,Carbonic anhydrase 9
UniProtID :Q16790
MASS(da) :49,698
MW(kDa) :46,50,58KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Carbonic Anhydrase 9 (CA9/G250).
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HT-29 cells
Function : Catalyzes the interconversion between carbon dioxide and water and the dissociated ions of carbonic acid (i.e. bicarbonate and hydrogen ions)..
Tissue specificity :Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.
Subcellular locationi :Nucleus. Nucleus, nucleolus. Cell membrane,Single-pass type I membrane protein. Cell projection, microvillus membrane,Single-pass type I membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.