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Western blot(SDS PAGE) analysis of extracts from SP2/0 cells.Using TCEA1 Rabbit mAb [Y8AP] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TCEA1,GTF2S,TFIIS,TCEA1_HUMAN,Transcription elongation factor A protein 1
UniProtID :P23193
MASS(da) :33,970
MW(kDa) : 40KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit monoclonal IgG
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human TCEA1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :SP2/0 cells
Function : Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.