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Western blot(SDS PAGE) analysis of extracts from(1) K562 cell lysate; (2) Jurkat cell lysate; (3) HeLa cell lysate; (4) 293T cell lysate .Using beta I Tubulin Rabbit mAb [3hSd]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Beta tubulin 1, class VI;Class VI beta tubulin antibody;TBB1_HUMAN;TUBB1;Tubulin beta 1 class VI;Tubulin, beta 1 antibody
UniProtID :Q9H4B7
MASS(da) :50,327
MW(kDa) :50kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat Cow Dog Monkey
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human beta I Tubulin
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected : (1) K562 cell lysate; (2) Jurkat cell lysate; (3) HeLa cell lysate; (4) 293T cell lysate
Function : Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity)..
Tissue specificity :Hematopoietic cell-specific. Major isotype in leukocytes, where it represents 50% of all beta-tubulins..
Subcellular locationi :Cytoplasm, cytoskeleton.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.