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beta I Tubulin Rabbit mAb [3hSd]Cat NO.: A59768

Western blot(SDS PAGE) analysis of extracts from(1) K562 cell lysate; (2) Jurkat cell lysate; (3) HeLa cell lysate; (4) 293T cell lysate .Using beta I Tubulin Rabbit mAb [3hSd]at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :Beta tubulin 1, class VI;Class VI beta tubulin antibody;TBB1_HUMAN;TUBB1;Tubulin beta 1 class VI;Tubulin, beta 1 antibody

UniProtID :Q9H4B7

MASS(da) :50,327

MW(kDa) :50kDa

Form :Liquid

Purification :Affinity-chromatography

Host :Rabbit

Isotype : IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat Cow Dog Monkey

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human beta I Tubulin

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected : (1) K562 cell lysate; (2) Jurkat cell lysate; (3) HeLa cell lysate; (4) 293T cell lysate

Function : Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity)..

Tissue specificity :Hematopoietic cell-specific. Major isotype in leukocytes, where it represents 50% of all beta-tubulins..

Subcellular locationi :Cytoplasm, cytoskeleton.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.