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Western blot(SDS PAGE) analysis of extracts from (1) 293T cell lysate; (2) C6 cell lysate.Using KRAS+HRAS+NRAS Rabbit mAb [pUDw]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :GTPase NRas; Transforming protein N-Ras; NRAS; HRAS1; GTPase HRas; H-Ras-1; Ha-Ras; Transforming protein p21; c-H-ras; p21ras; HRAS; HRAS1; GTPase KRas; K-Ras 2; Ki-Ras; c-K-ras; c-Ki-ras; KRAS; KRAS2; RASK2;
UniProtID :P01111/P01112/P01116
MASS(da) :21,229
MW(kDa) :21kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human KRAS+HRAS+NRAS
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1) 293T cell lysate; (2) C6 cell lysate.
Function : Ras proteins bind GDP/GTP and possess intrinsic GTPase activity..
Subcellular locationi :Cell membrane,Lipid-anchor,Cytoplasmic side. Golgi apparatus membrane,Lipid-anchor.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.